Which of the following is NOT used to generate new glucose via gluconeogenesis?

In gluconeogenesis, the body synthesizes glucose from non-carbohydrate sources. Lactate, amino acids, and glycerol can all serve as precursors for this metabolic pathway, but acetyl CoA does not.

Lactate can be converted back into pyruvate through the Cori cycle, where it is then transformed into glucose. Similarly, certain amino acids, particularly glucogenic ones, can be converted into intermediates of the citric acid cycle, ultimately leading to glucose production. Glycerol, derived from triglyceride breakdown, can enter gluconeogenesis after being converted to dihydroxyacetone phosphate (DHAP), a glycolytic intermediate.

Acetyl CoA, however, cannot give rise to glucose in gluconeogenesis because it is the end product of fatty acid oxidation and is primarily involved in energy production rather than glucose synthesis. When acetyl CoA enters the citric acid cycle, it contributes to ATP production, but it does not provide amino acid carbon skeletons that are required for gluconeogenesis. This is primarily due to the fact that the conversion of two molecules of acetyl CoA to one molecule of glucose would not be possible since the carbon atoms from acetyl CoA are released as CO